1. HPLC Column Care  Receipt of New Column  Tubing and Fittings  Connecting Tubing Fittings  Manufacturer’s Fittings  Connecting Fittings  SecurityLink  SecurityGuard  Mobile Phase  Other Considerations  Column Protection  Guard Columns  SecurityGuard™ Column  Cleaning Column  Storage Column Care  Summary 2. HPLC Troubleshooting  Troubleshooting  Preventive Maintenance  Diagnosing Problems  Instrument vs. Column  Pressure Problems  Gradient Problems  Detector Problems  Variable Retention Times  Peak Shape is Critical  Column Frits  Peak Tailing  All Peaks Tail  Peak Fronting  Peak Splitting  Broad/Symmetrical Peaks  Ghost Peaks  Decreased Column Performance  Troubleshooting Summar Questions?