Resolution: The Goal of Liquid Chromatography Part 1. Chemical and Physical Properties 분석물질(제약성분) 정보(이름 또는 구조) 만으로, 기시법이나 논문 자료 없이, 정량 분석의 메소드 신속(5 분) 설정 및 기시법 검증 방법. 역상 분리 메커니즘 _ 분리가 진행되는 근본 원리, 왜? Part 2. The Evolution of Core-Shell HPLC HPLC의 발전 과정을 통하여 향후 5 ~ 10 년 발전 방향 예상 및 고효율의 장점 Part 3. Reversed Phase Column Selectivity 분석법 컨설팅 담당자의 시각으로 본(분리능, 감도, 분석시간 등 개선을 위한) 분석물질과 컬럼 매칭 방법. 왜 다양한 종류의 고정상 컬럼이 필요한가? 각 고정상 컬럼의 용도 Part 1. Chemical and Physical Properties  기존 HPLC 분석법 개발 방법  스마트 HPLC 분석법 개발 방법  컬럼피아 _ Columnpia AI RAG  Liquid Chromatographic Process  HPLC Method Development  Physicochemical Properties  What is Lipophilicity? Log P _ Partition Coefficient  Effect of pH on Solute ionization. Log D _ Distribution Coefficient  Retaining Compounds  Mechanisms of Interaction in RP Chromatography  Profiling Mechanisms of Reversed Phase Selectivity  Binding Energy by Retention Mechanism  Resolution: The Goal of Liquid Chromatography Part 2. The Evolution of Core-Shell HPLC  The Evolution of Core-Shell  Particle Morphology _ Core Shell  Chromatographic Efficiency  The Core-Shell Advantage  Chromatographic Efficiency  Impact of Efficiency on Performance  Scalability and Portability  Ultra-High Performance on Any LC System  Choosing the Right Core-Shell Particle  Core Shell Stationary Phases  How Resolution is Affected  Narrow Particle Size Distribution (Lower “A” Term)  Advantages of Ultra-High Efficiency  Evolution of LC Particle Technology  Master Resolution Equation Part 3. Reversed Phase Column Selectivity  Chromatography Media  Review HPLC Media  Fully porous silica  Core-Shell particles  Monolithic rods  Organosilica hybrid  Phenomenex Column Portfolio Overview  Mechanisms of Interaction in RP Chromatography  Binding Energy by Retention Mechanism  Stationary Phase Selectivity (Column) Selection  Alkyl Bonded Phases  Hydrophobic Selectivity  Phenyl-Bonded Phases  Aromatic Based Selectivity  Polar-Modified Phases  Polar Embedded / End capped Phase  Polar Basic Compounds  Optimizing Column Selectivity with Analyte Chemistry  Polar Interactions – Potential for Hydrogen Bonding  Hydroxyl- or Amine- Containing Compounds  Non-Ionized Bases and Oxygen or Halogen- Containing Compounds * Special Part 1  Reversed=Phase Solvents  Solvent Considerations  Solvent Strength: Acetonitrile versus Methanol  Solvent Selectivity for Phenyl Phase  Buffer Selection for RP-HPLC  Effect of pH on Analyte Retention Behavior  Solvent Considerations for Method Development * Special Part 2  Fittings  Manufacturer’s Fittings  Connecting Fittings  SecurityLink  SecurityGuard  Variable Retention Times  Peak Shape is Critical  Column Frits  Ghost Peaks  Troubleshooting Summary