Implementing Both for Better HPLC and UHPLC Results 1. Kinetex Core-Shell Particle Selection 2. Core-Shell Performance Advantage Moving to core-shell particles: • Increase efficiency • Increase resolution • Increase sensitivity • Less retention (usually) • Pressure is equivalent to fully porous • Improved performance without the pressure penalty 3. Kinetex Stationary Phases – Alkyl 4. Kinetex Stationary Phases – Orthogonal 5. Luna Omega Particle Selection 6. Highly Inert Surface 7. Luna Omega Selectivity Options 8. Fully Porous vs Core-Shell 9. Increased Retention of Luna Omega Stationary Phase Selectivities 1. Polar Bases at “Typical” pH Range Kinetex Biphenyl: 1. Aromatic, pi-pi interactions 2. Weak dipole interactions 3. Hydrophobic interactions 4. Hydrogen bonding Polar C18 chemistry: 1. C18 with proprietary polar surface modification 2. Hydrophobic interactions + 100% Aqueous stability 3. Enhanced polar retention balanced with non-polar selectivity 4. Core-Shell and Thermally Modified FP Options 2. Polar Selectivity of Polar C18 3. Improved Peak Share for Bases at Low pH PS C18 chemistry: 1) C18 phase with TMS endcapping 2) Secondary surface modification with positively charged functional group 3) Improved peak shape and loadability for basic molecules 4) Increased selectivity for polar acids 4. Improved Peak Shape for Bases  Improved loading for impurity profiling for basic compounds  Selectivity and Resolution maintained with increasing impurity concentration 5. Kinetex F5: Unique Selectivity F5 chemistry: A. Hydrophobic - Carbon in linker and ring encourages neutral/hydrophobic retention B. Aromatic – π-π interactions between phenyl ring and analyte π electrons results in increased retention C. Electrostatic - Highly electronegative fluorine groups create dipole moments, aiding in polar compound retention D. Steric/Planar - Shape selectivity allows for isomeric separations that are not possible on traditional alkyl phases E. Hydrogen Bonding - Polar functional groups of analyte interact with the electronegative fluorine 6. Selectivity of F5: Positional Isomers 7. Product Scorecard General Chromatographic Performance System Considerations Method Development/Applications